Measuring Gene Expression

Book Summary

In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including RT-PCR, differential display, RNA interference, reporter genes, microarrays, and proteomics, as well as a section on RNA isolation and analysis. There is an overview of each method: its pros and cons, sample preparation, sources of error, and data interpretation.

1. Gene Expression and its Control  2. Isolation and Analysis of RNA  3. Hybridization-based Methods for Measuring Transcript Levels  4. PCR-based Methods for Measuring Transcript Levels  5. Differential Display, Subtractive Hybridization, Amplification Suppression and SAGE Techniques for Measuring Gene Expression  6. Measuring Gene Expression Using Reporter Gene Assays  7. Analysis of the Proteome  8. Statistical Analysis of Gene Expression Data