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Real-time PCR

Edition:
1st
Author(s):
ISBN:
9780415377348
Format:
Paperback
Publication Date:
April 27, 2006
Content Details:
362 pages
Language:
English

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  • About the Book

    Book Summary

    With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

    Features

    • Provides a firm grounding in the principles of real-time PCR and allows those who wish to, to develop further applications.
    • Each chapter conveys the invaluable hands on experience of those using the technique.
    • Covers both general and specialized uses of real-time PCR.

    Reviews

      The goal is to provide both theoretical and practical information for those wishing to use real time PCR as a laboratory tool. This is a generally worthwhile aim and, with some caveats, suitably met. This is a useful book in that limitations and uses are both covered. Doody's Notes, February 2007

  • Contents

    1. An Introduction to Real-time PCR  2. Data Analysis and Reporting  3. Relative Quantification  4. Normalisation  5. High-Throughput Primer and Probe Design  6. Quantitative Analysis of Ocular Gene Expression  7. Quantitative Gene Expression by Real-time PCR  8. Real-time PCR Using SYBR Green  9. High-Resolution Melting Analysis for Scanning and Genotyping  10. Quantitative Analyses of DNA Methylation  11. Mitochondrial DNA Analysis  12. Real-time Immuno-PCR  13. Clinical Microbiology  14. Clinical Virology  15. Solid Organ Transplant Monitoring  16. Real-time PCR Applications in Haematology  17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies