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Enzyme Kinetics and Mechanism

Edition:
1st
Author(s):
Paul F. Cook, W. W. Cleland
ISBN:
9780815341406
Format:
Hardback
Publication Date:
March 06, 2007
Content Details:
416 pages
Language:
English

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  • About the Book

    Book Summary

    Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics.  Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. 

    Practical examples taken from the literature demonstrate theory throughout.  The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. 

    Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level.  Enzymologists and molecular biologists will find the text a useful reference.

    Features

      • Includes a comprehensive treatment of enzyme kinetics.
      • Explains the underlying kinetic behavior for given mechanisms.
      • Replete with examples of theory in practice and current research in literature.
      • Authored by two of the premier practitioners in the field of enzyme kinetics.
  • Contents

    1. Nomenclature  
    2. Introduction to Kinetics 
    3. Enzyme Assays  
    4. Derivation of Initial Velocity Rate Equations and Data Processing  
    5. Initial Velocity Studies in the Absence of Added Inhibitors  
    6. Initial Velocity Studies: Presence of Added Inhibitors  
    7. Pre-Steady State and Relaxation Kinetics  
    8. Isotopic Probes of Kinetic Mechanism  
    9. Isotope Effects as a Probe of Mechanism  
    10. pH Dependence of Kinetic Parameters and Isotope Effects 
    Appendix I. King and Altman Patterns and Distribution Equations 
    Appendix II. Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations and Rate Constants for a Number of Multireactant Mechanisms

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