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Real-Time PCR

An Essential Guide

Publication Date:
April 15, 2004
Content Details:
300 pages

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  • About the Book

    Book Summary

    This essential manual presents a comprehensive guide to the most appropriate and up-to-date technologies and applications as well as providing an overview of the theory of this important technique. Written by recognized experts in the field this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: Real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection.


      The book is well laid out and is easy to read due to its clear presentation style...this book presents such a wealth of information on the topics of real-time PCR that it would be of great use to anyone working in the field or interested in the topic. - Austrialian Journal of Medical Science, February 2005; vol 26; no. 1

      This book represents excellent value for libraries and individuals or groups that already perform or are considering real-time PCR. - Microbiology Today, Vol. 31. November 2004

      Real-Time PCR: An Essential Guide is an extremely useful volume with a great deal of helpful information. It would be invaluable to any lab or research center planning to buy a system and start using real-time PCR, and would be a good addition to any biology or medical library that has resources for molecular research. - E-Streams, Vol 8, No. 5, May 2005

  • Contents

    1. An Introduction to Real-Time PCR  2. An Overview of Real-Time PCR Platforms  3. Homogeneous Fluorescent Chemistries for Real-Time PCR  4. Performing Real-Time PCR  5. Internal and External Controls for Reagent Validation  6. Quantitative Real-Time PCR  7. Analysis of mRNA Expression by Real-Time PCR 8. Mutation Detection by Real-Time PCR 9. The Quantitative Amplification Refractory Mutation System  10. Real-Time NASBA  11. Applications of Real-Time PCR in Clinical Microbiology  12. Application of Real-Time PCR to the Diagnosis of Invasive Fungal Infection