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A Laboratory Course in Biomaterials

Wujing Xian
Publication Date:
June 18, 2009
Content Details:
216 pages

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  • About the Book

    Book Summary

    The field of biomedical engineering has vastly expanded in the past two decades, as reflected in the increased number of bioengineering and biomaterials programs at universities. The growth of this area has outpaced the development of laboratory courses that allow students hands-on experience, since the barriers involved in creating multidisciplinary biomaterials laboratory courses are high. A Laboratory Course in Biomaterials provides a teaching tool comprehensive in scope perspective.

    Multidisciplinary approach

    Suitable for junior or senior level laboratory courses in biomaterials and bioengineering, this volume trains students in laboratory skills, data analysis, problem solving, and scientific writing. The text takes a multidisciplinary approach, integrating a variety of principles that include materials science, chemistry, biochemistry, molecular and cell biology, and engineering.

    Step-by-step instructions

    The author presents flexible modules that allow the coursework to be adapted to the needs of different departments. Each module is organized around a central theme, such as drug delivery and natural biomaterials, to enhance student comprehension. This book provides step-by-step descriptions of lab procedures, reagents, equipment, and data processing guidelines. It also includes a series of thought-provoking questions and answers following each experiment, drawn from the author’s own experience in teaching a biomaterials laboratory course at the University of Illinois.

    Timely in its coverage, many of the experiments presented in the book are adapted from research papers reflecting the progress in various disciplines of bioengineering and biomaterials science.


      • Presents detailed, step-by-step descriptions of lab procedures and explanations for the underlying principles
      • Contains user-friendly graphic illustrations for the experiments
      • Provides a series of questions and answers at the end of each chapter to clarify concepts and induce critical thinking
      • Includes text boxes with in-depth information, case studies, and lab tips
      • Offers information on equipment, reagents, and supplies for instructors
  • Contents

    Basic Laboratory Skills

    Commonly Used Lab Equipment and Supplies

    Waste Disposal

    Liquid Transfer


    Making Solutions

    Error Analysis

    Module I. Drug Delivery: Controlled Release of Encapsulated Protein from PLGA Microspheres

    Session 1. Encapsulation of BSA in PLGA Microspheres

    Session 2. Evaluation of the Encapsulation Efficiency and the Drug Load

    Session 3. Controlled Release of BSA from PLGA Microspheres

    Session 4. Evaluation of Controlled Release Profile


    Appendix. Recipes and Sources for Equipment, Reagents, and Supplies

    Module II. Natural Biomaterials: Collagen and Chitosan

    Session 1. Extracting Acid-Soluble Type I Collagen from Bovine Calf Skin

    Session 2. Purification of Extracted Collagen

    Session 3. Analysis of Collagen Extraction and Purification by Electrophoresis

    Session 4. Fabricating Collagen/Chitosan Sponges


    Appendix. Recipes and Sources for Equipment, Reagents and Supplies

    Basic Laboratory Skills II. Cell Culture and Microscopy

    Cell Culture


    Appendix. Sources and Recipes for Equipment, Reagents, and Supplies

    Module III. Biocompatibility Testing: Cytotoxicity and Adhesion

    Session 1. Cytotoxicity Evaluation Using Direct Contact Tests

    Session 2. Cytotoxicity Evaluation Using Liquid Extracts of Materials

    Session 3. Biofunctionality Evaluation through Cell Adhesion/Proliferation Tests


    Appendix. Recipes and Sources for Equipment, Reagents and Supplies

    Module IV. Tissue Engineering: Organotypic Cultures of Skin Equivalents

    Session 1. The Dermis Equivalent

    Session 2. The Epidermis Equivalent

    Session 3. Histological Studies of Natural Skin and Organotypic Skin Equivalent


    Appendix A. Sterilizing Collagen and Preparing Collagen Solution

    Appendix B. Custom-Made Teflon Support for Air Exposure of the Epidermis Equivalent

    Appendix C. Recipes and Sources for Equipment, Reagents and Supplies

    Module V. Bioceramics: Porous Hydroxyapatite Composite

    Session 1. Synthesis of HA and FA

    Session 2. X-Ray Powder Diffraction of HA and FA

    Session 3. Fabrication of Porous HA/FA-PLGA Composite Using a Salt-Leaching Method

    Session 4. Characterization of the Porous HA/FA-PLGA Composites—Acid Degradation and Compression Testing


    Appendix. Recipes and Sources for Equipment, Reagents and Supplies

    Appendix: Answers